![SOLVED: QNo.3. Calculate Tm of the following primers: Gene: Sequence Melting temperature Forward primer: TCGAGTCGCGTCCACC Reverse primer: GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR = Cytogenetic location = SOLVED: QNo.3. Calculate Tm of the following primers: Gene: Sequence Melting temperature Forward primer: TCGAGTCGCGTCCACC Reverse primer: GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR = Cytogenetic location =](https://cdn.numerade.com/ask_images/479db41e130a4d759330f82f59f9f3f4.jpg)
SOLVED: QNo.3. Calculate Tm of the following primers: Gene: Sequence Melting temperature Forward primer: TCGAGTCGCGTCCACC Reverse primer: GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR = Cytogenetic location =
![Figure 1 from Controlled hot start and improved specificity in carrying out PCR utilizing touch-up and loop incorporated primers (TULIPS). | Semantic Scholar Figure 1 from Controlled hot start and improved specificity in carrying out PCR utilizing touch-up and loop incorporated primers (TULIPS). | Semantic Scholar](https://d3i71xaburhd42.cloudfront.net/99913474d2cc168c4f3ea76004c7fafd02fbbef6/1-Figure1-1.png)
Figure 1 from Controlled hot start and improved specificity in carrying out PCR utilizing touch-up and loop incorporated primers (TULIPS). | Semantic Scholar
![Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection | Scientific Reports Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection | Scientific Reports](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fsrep12543/MediaObjects/41598_2015_Article_BFsrep12543_Fig1_HTML.jpg)